embl embl emnew ensembl.cdna est_hum est_mam est_rod est_mus est_vrt est_pln est_inv est_fun est_pro gss_fun gss_hum gss_inv gss_pln gss_pro gss_mus gss_vrt gss_other htg_hum htg_inv htg_rod htg_mus htg_pln htg_other htgo_hum htgo_inv htgo_mus htgo_other sts emvec igvec imgtligm imgthla BLOSUM62 BLOSUM62 BLOSUM80 PAM30 PAM70 10.0 0 0 1 2 3 4 5 6 7 8 9 0 0 true 0 -3 1 250 500 F F T 0 0 false 0.0 3 1 1

Query sequence in a fasta format embl Database blastn Program BLOSUM62 Matrix 10.0 Expectation value (E) 0 Alignment view options 0 Cost to open a gap 0 Cost to extend a gap true Perfom gapped alignment 0 X dropoff value for gapped alignment -3 Penalty for a nucleotide mismatch 1 Reward for a nucleotide match 250 Number of alignments to show 500 Number of one-line descriptions to show F Filter query sequence 0 Threshold for extending hits 0 Word size false Use lower case filtering of FASTA sequence 0.0 Dropoff (X) for blast extensions in bits 3 Query strands to search against database [3 is both, 1 is top, 2 is bottom] 1 Query genetic code 1 Database genetic code