restrict European Bioinformatics Institute http://www.ebi.ac.uk/soaplab/emboss4/services/nucleic_restriction.restrict http://www.ebi.ac.uk/soaplab/emboss4/services/nucleic_restriction.restrict?wsdl Finds restriction enzyme cleavage sites. Detailed info about this operation can be found at the following link: http://emboss.sourceforge.net/apps/release/4.0/emboss/apps/restrict.html restrict Finds restriction enzyme cleavage sites. Detailed info about this operation can be found at the following link: http://emboss.sourceforge.net/apps/release/4.0/emboss/apps/restrict.html sequence_usa Type: string. DNA sequence. The Uniform Sequence Address, or USA, is a standard way of specifying a sequence to be read into a program in EMBOSS. The most common ways of specifying a sequence is to type (database:entry), where database can be embl, uniprot or swissprot and entry is either the sequence`s entry or ID name, or its Accession number in that database. For example, database:accession -->embl:X65923 or swissprot:Q7M4G0 | database:entry_name -->swissprot:AMIC_PSEAE |database:ID_name -->embl:paamir. Choose either this parameter or the sequence_direct_data parameter but not both together. Parameter mandatory if the sequence_direct_data parameter has not been chosen. false http://www.mygrid.org.uk/ontology#EMBL_accession String sequence_direct_data Type: string. DNA sequence. Choose this parameter if you want to provide the 'sequence' value as string (DNA sequence). Parameter mandatory if the sequence_usa parameter hasn't been chosen. false http://www.mygrid.org.uk/ontology#DNA_sequence http://www.mygrid.org.uk/ontology#single_sequence_format String sformat Type: string. Specifies the format of the input sequence. This is an optional parameter. The allowed values for this parameter are: gcg, gcg8, embl, swiss, fasta, ncbi, genbank, nbrf, pir, codata, strider, clustal, phylip, acedb, msf, jackknifer, jackknifernon, nexus, nexusnon, treecon, mega, meganon, ig, staden, text, raw. By default the service autodetects the sequence format. false String sbegin Type: long. The first position to be used in the sequence, for example: sbegin=1 (default value) means start with the first base in the sequence. This is an optional parameter. false String send Type: long. The last position to be used in the sequence. This is an optional parameter. By default it is the end the sequence. false String sprotein Type: boolean. Is the sequence a protein? This is an optional parameter. By default the service can autodetect if a sequence is a nucleotide or protein sequence. false String snucleotide Type: boolean. Is the sequence a nucleotide? This is an optional parameter. By default the service can autodetect if a sequence is a nucleotide or protein sequence. false String sreverse Type: boolean. Use reverse complement of the nucleic acid sequence. This is an optional parameter. False by default. false String slower Type: boolean. Convert the sequence to lower case. This is an optional parameter. False by default. false String supper Type: boolean. Convert the sequence to UPPER case. This is an optional parameter. False by default. false String sitelen Type: long. This sets the minimum length of the restriction enzyme recognition site. Any enzymes with sites shorter than this will be ignored. This is an optional parameter. The default is 4 4 false String enzymes Type: string. Comma separated enzyme list. For example: 'HincII,hinfI,ppiI,hindiii'. The name 'all' reads in all enzyme names from the REBASE database. This is an optional parameter. The default is 'all' all false http://www.mygrid.org.uk/ontology#REBASE_record String datafile_direct_data Type: string. Restriction enzyme data. This is an optional parameter. false String datafile_url Type: string. URL of the restriction enzyme data. This is an optional parameter. false String min Type: long. This sets the minimum number of cuts for any restriction enzyme that will be considered. Any enzymes that cut fewer times than this will be ignored. This is an optional parameter. The default is 1. 1 false String max Type: long. This sets the maximum number of cuts for any restriction enzyme that will be considered. Any enzymes that cut more times than this will be ignored. This is an optional parameter. The default is 2000000000. 2000000000 false String solofragment Type: boolean(true/false). This gives the fragment lengths of the forward sense strand produced by complete restriction by each restriction enzyme on its own. Results are added to the tail section of the report. This is an optional parameter. The default is 'false'. false false String single Type: boolean (true/false). If this is set then this forces the values of the mincuts and maxcuts qualifiers to both be 1. Any other value you may have set them to will be ignored. This is an optional parameter. The default is 'false'. false false String blunt Type: boolean (true/false). This allows those enzymes which cut at the same position on the forward and reverse strands to be considered. This is an optional parameter. The default is 'true'. true false String sticky Type: boolean (true/false). This allows those enzymes which cut at different positions on the forward and reverse strands, leaving an overhang, to be considered. This is an optional parameter. The default is 'true'. true false String ambiguity Type: boolean (true/false). This allows those enzymes which have one or more 'N' ambiguity codes in their pattern to be considered. This is an optional parameter. The default is 'true'. true false String plasmid Type: boolean (true/false). If this is set then this allows searches for restriction enzyme recognition site and cut positions that span the end of the sequence to be considered. This is an optional parameter. The default is 'false'. false false String commercial Type: boolean (true/false). If this is set, then only those enzymes with a commercial supplier will be searched for. This qualifier is ignored if you have specified an explicit list of enzymes to search for, rather than searching through 'all' the enzymes in the REBASE database. It is assumed that, if you are asking for an explicit enzyme, then you probably know where to get it from and so all enzymes names that you have asked to be searched for, and which cut, will be reported whether or not they have a commercial supplier. This is an optional parameter. The default is 'true'. true false String limit Type: boolean. This limits the reporting of enzymes to just one enzyme from each group of isoschizomers. If this value is set to be false then all of the input enzymes will be reported. You might like to set this to false if you are supplying an explicit set of enzymes rather than searching 'all' of them. This is an optional parameter. The default is 'true'. true false String alphabetic Type: boolean (true/false). If you use this option, then you will get a single list of the program names and descriptions instead of the programs being listed in their functional groups. This is an optional parameter. The default is 'false'. false false String fragments Type: boolean. This gives the fragment lengths of the forward sense strand produced by complete restriction using all of the input enzymes together. Results are added to the tail section of the report. This is an optional parameter. The default is 'false'. false false String name Type: boolean (true/false). Show sequence name. The default is 'false'. false false String report Type: string. A general report for the underlying analysis job. false String detailed_status Type: long. The exit code of the underlying analysis job, 0 means the job returned normally. String outfile Returns the base number, restriction enzyme name, recognition site and cut positions. false http://www.mygrid.org.uk/ontology#restriction_enzyme_report String http://www.mygrid.org.uk/ontology#predicting http://www.mygrid.org.uk/ontology#EMBL_nucleotide_sequence_database Soaplab service