dan European Bioinformatics Institute http://www.ebi.ac.uk/soaplab/emboss4/services/nucleic_composition.dan http://www.ebi.ac.uk/soaplab/emboss4/services/nucleic_composition.dan?wsdl Calculates DNA RNA/DNA melting temperature. Detailed info about this operation can be found at the following link: http://emboss.sourceforge.net/apps/release/5.0/emboss/apps/dan.html dan Calculates DNA RNA/DNA melting temperature. Detailed info about this operation can be found at the following link: http://emboss.sourceforge.net/apps/release/5.0/emboss/apps/dan.html sequence_usa Type: string. DNA sequence. The Uniform Sequence Address, or USA, is a standard way of specifying a sequence to be read into a program in EMBOSS. The most common ways of specifying a sequence is to type (database:entry), where database can be embl, uniprot or swissprot and entry is either the sequence`s entry or ID name, or its Accession number in that database. For example, database:accession -->embl:X65923 or swissprot:Q7M4G0 | database:entry_name -->swissprot:AMIC_PSEAE |database:ID_name -->embl:paamir. Choose either this parameter or the sequence_direct_data parameter but not both together. Parameter mandatory if the sequence_direct_data parameter has not been chosen. false String sequence_direct_data Type: string. DNA or RNA sequence. Choose this parameter if you want to provide the 'sequence' value(DNA or RNA sequence) as string. Parameter mandatory if the sequence_usa parameter hasn't been chosen. false http://www.mygrid.org.uk/ontology#nucleotide_sequence http://www.mygrid.org.uk/ontology#single_sequence_format String sformat Type: string. Specifies the format of the input sequence. This is an optional parameter. The allowed values for this parameter are: gcg, gcg8, embl, swiss, fasta, ncbi, genbank, nbrf, pir, codata, strider, clustal, phylip, acedb, msf, jackknifer, jackknifernon, nexus, nexusnon, treecon, mega, meganon, ig, staden, text, raw. By default the service autodetects the sequence format. false String sbegin Type: long. The first position to be used in the sequence, for example: sbegin=1 (default value) means start with the first base in the sequence. This is an optional parameter. false String send Type: long. The last position to be used in the sequence. This is an optional parameter. By default it is the end the sequence. false String sprotein Type: boolean. Is the sequence a protein? This is an optional parameter. By default the service can autodetect if a sequence is a nucleotide or protein sequence. false String snucleotide Type: boolean. Is the sequence a nucleotide? This is an optional parameter. By default the service can autodetect if a sequence is a nucleotide or protein sequence. false String sreverse Type: boolean. Use reverse complement of the nucleic acid sequence. This is an optional parameter. False by default. false String slower Type: boolean. Convert the sequence to lower case. This is an optional parameter. False by default. false String supper Type: boolean. Convert the sequence to UPPER case. This is an optional parameter. False by default. false String windowsize Type: long(from 1 to 100). The values of melting point and other thermodynamic properties of the sequence are determined by taking a short length of sequence known as a window and determining the properties of the sequence in that window. The window is incrementally moved along the sequence with the properties being calculated at each new position. This is a mandatory parameter. The default is 20 20 false String shiftincrement Type: long. This is the amount by which the window is moved at each increment in order to find the melting point and other properties along the sequence. This is a mandatory parameter. The default is 1. 1 false String dnaconc Type: float. Enter DNA concentration (nM). This is a mandatory parameter. The default is 50.0 50.0 false String saltconc Type: float. Enter salt concentration (mM). This is a mandatory parameter. The default is 50.0 50.0 false String graph_format Type: string. Format of the graphical output. This is an optional parameter. The allowed values for this parameter are: png, postscript, colourps, hpgl. The default is png. png false String product Type: boolean(Yes/No). This prompts for percent formamide, percent of mismatches allowed and product length. This is an optional parameter. The default is 'No'. false String formamide Type: float. This specifies the percent formamide to be used in calculations (it is ignored unless the 'product' parameter is used). This is an optional parameter. The default is 0.0 0.0 false String mismatch Type: float. This specifies the percent mismatch to be used in calculations (it is ignored unless the 'product' parameter is used). This is an optional parameter. The default is 0.0 0.0 false String prodlen Type: long. This specifies the product length to be used in calculations (it is ignored unless the 'product' parameter is used). This is an optional parameter. The default is 20. false String thermo Type: boolean (Yes/No). Output the DeltaG, DeltaH and DeltaS values of the sequence windows to the output data file. This is an optional parameter. The default is 'No' false String temperature Type: float. If the 'thermo' parameter has been specified then this specifies the temperature at which to calculate the DeltaG, DeltaH and DeltaS values. This is an optional parameter. The default is 25.0 25.0 false String rna Type: boolean(Yes/No). This specifies that the sequence is an RNA sequence and not a DNA sequence. This is an optional parameter. The default is 'No'. false false String plot Type: boolean(Yes/No). If this is not specified then the file of output data is produced, else a plot of the melting point along the sequence is produced. This is an optional parameter. The default is 'No'. false String mintemp Type: float. Enter a minimum value for the temperature scale (y-axis) of the plot. This is an optional parameter. The default is 55.0 55.0 false String report Type: string. A general report for the underlying analysis job. false String detailed_status Type: long. The exit code of the underlying analysis job, 0 means the job returned normally. false String outfile Returns DNA RNA/DNA melting temperature. false String Graphics_in_Postscript Plot the melting point along the sequence in Postscript format. false String Graphics Plot the melting point along the sequence false byte[] Graphics_in_PNG Plot the melting point along the sequence in png format. false byte[][] http://www.mygrid.org.uk/ontology#calculating Soaplab service